𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Scintillation Proximity Assay for UDP-GalNAc:Polypeptide,N-Acetylgalactosaminyltransferase

✍ Scribed by C.A. Baker; R.A. Poorman; F.J. Kézdy; D.J. Staples; C.W. Smith; Å.P. Elhammer


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
103 KB
Volume
239
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


A Scintillation Proximity Assay for RNA
✍ Jianwei Liu; Patricia A. Feldman; Jonathan S. Lippy; Ekaterina Bobkova; Michael 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 101 KB

A homogeneous scintillation proximity assay (SPA) for detection of RNA transcripts is described. 3H-labeled RNA transcripts are hybridized in solution to biotinylated oligodeoxynucleotides (ODNs), which are then bound by streptavidin-coated, scintillant-embedded beads. Only bound 3H-labeled RNA tran

A Scintillation Proximity Assay for Poly
✍ Anissa Cheung; Jie Zhang 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 199 KB

Poly(ADP-ribose) polymerase (PARP) is an abundant nuclear protein in most of the eukaryotic tissues. When activated by DNA damage, PARP synthesizes poly(ADP-ribose) from NAD. Conventional radioactive PARP enzyme assay requires the separation of the polymer product from the NAD substrate, a rate-limi

A Fluorescent Assay for the Determinatio
✍ C.A. Palmerini; A. Datti; S. Alunni; I.E. Vanderelst; A. Orlacchio 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 429 KB

A fluorescent assay for UDP-GlcNAc: Galp1,3GalNAc-R \(\boldsymbol{\beta 1 , 6 - \boldsymbol { N } \text { -acetylglucosaminyltransferase (core } 2}\) GlcNAc-T) activity has been developed involving dansylation of the enzyme reaction product. Core 2 GlcNAc-T detection was performed using unlabeled UD

Development of a Scintillation Proximity
✍ Xin He; John P. Mueller; Kevin A. Reynolds 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 170 KB

Assays of ␤-ketoacyl-acyl carrier protein synthases III (KASIII; FabH), a key enzyme initiating bacterial type II fatty acid biosynthesis, usually involve incubation of radiolabeled acetyl-coenzyme A and malonylacyl carrier protein (MACP). The radiolabeled acetoacetyl-ACP product is precipitated and