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A scanning electron microscopic and X-ray microanalytic study of cell surface material during amphibian neurulation

✍ Scribed by Rice, Robert W. ;Moran, Denis J.

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
536 KB
Volume
201
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Treatment with lanthanum (La^3+^) after fixation in phosphate (PO~4~^−3^)‐buffered glutaraldehyde results in the deposition of a cell surface material (CSM) primarily on the developing urodele amphibian neural axis. X‐ray probe microanalysis indicates that calcium (CA^2+^) levels are considerably higher in the neural fold region. La^3+^ displaces Ca^2+^ from negatively‐charged moieties on biological membranes. Once bound, La^3+^ likely interacts with residual phosphate(s) resulting in deposition of CSM. Elemental X‐ray microanalysis shows CSM contains mostly lanthanum and phosphorus. The high level of regional La^3+^ binding is correlated with inherently greater Ca^2+^ levels in the developing neural axis.

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