A role for decorin in controlling proliferation, adhesion, and migration of murine embryonic fibroblasts

✍ Scribed by Z. Ferdous; S. B. Peterson; H. Tseng; D. K. Anderson; R. V. Iozzo; K. J. Grande-Allen

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 574 KB
• Volume: 9999A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32545

✦ Synopsis

Abstract

The proteoglycan decorin putatively inhibits cell adhesion and cell migration on various extracellular matrix substrates through interactions with β~1~ integrins. This study, therefore, examined the adhesive, migration, and proliferative characteristics of decorin knockout (Dcn^−/−^) murine embryonic fibroblasts compared to wild‐type controls on collagen‐coated, fibronectin‐coated, and uncoated tissue culture plates. The Dcn^−/−^ cells showed significantly greater proliferation than wild‐type controls on all substrates. The Dcn^−/−^ cells also showed significantly greater adhesion to both collagen and fibronectin; both cell types showed greater adhesion to collagen. The addition of exogenous decorin had a differential effect on adhesion to collagen between cell types, but not on fibronectin. For collagen, blocking either α~2~ or β~1~ integrin subunits significantly reduced adhesion for Dcn^−/−^ cells; whereas for fibronectin, blocking either the α~5~ or β~1~ integrin subunits reduced adhesion for both cell types. Decorin and the α~5~β~1~ integrin may have lesser roles in adhesion to fibronectin than previously presumed. Finally, compared to wild‐type cells, Dcn^−/−^ cells showed greater migration on both uncoated and collagen substrates. This study demonstrates that decorin affects the biology of various integrins that participate in cell proliferation, adhesion, and migration on various substrates. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010