## Abstract Since their derivation, human embryonic stem (hES) cells have been used for a variety of applications including developmental biology, pathology, chemical biology, genomics, and proteomics. However, their most important potential application is the generation of cells and tissues, which
A ROCK inhibitor permits survival of dissociated human embryonic stem cells
β Scribed by Wataya, Takafumi; Takahashi, Jun B; Nishikawa, Satomi; Nishikawa, Shin-ichi; Muguruma, Keiko; Watanabe, Kiichi; Ueno, Morio; Kamiya, Daisuke; Nishiyama, Ayaka; Matsumura, Michiru
- Book ID
- 109910950
- Publisher
- Nature Publishing Group
- Year
- 2007
- Tongue
- English
- Weight
- 534 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1310
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## Abstract Pluripotent human embryonic stem (hES) cells require mechanisms to maintain genomic integrity in response to DNA damage that could compromise competency for lineageβcommitment, development, and tissueβrenewal. The mechanisms that protect the genome in rapidly proliferating hES cells are
Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protect the cells is a prerequisite for developing effi