The location of genes in the vicinity of the major FP2 origin on the chromosome of Pseudomonas aeruginosa PAO has been revised. The markers hisl (a transduction group of histidine biosynthetic genes) and pru (a gene cluster encoding proline utilization functions) were located in the 90 to 95/0 min c
A revised map location for the histidine utilization genes in Pseudomonas putida
โ Scribed by Robin S. King; Lynne L. Sechrist; Dr. Allen T. Phillips
- Book ID
- 102390600
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 321 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0233-111X
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โฆ Synopsis
The histidine utilization genes hutH and hutU of Pseudomonusputidu ATCC 12633 have been mapped by interrupted mating and transduction to a location at approximately 43 minutes on the chromosome, closely linked to ser-800 and met-400 markers previously shown to be at 46 and 42 minutes, respectively. Since restriction enzyme mapping and cloning results have established that all genes associated with the hut pathway are contiguous, earlier maps of this strain which place these genes near 10 minutes on the chromosome in a superoperonic catabolic cluster are in error.
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