A review on the electron ionization and fast atom bombardment mass spectrometry of polyphenols naturally occurring in olive wastes and some of their synthetic derivatives
✍ Scribed by Renato Capasso
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 126 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Electron ionization and fast atom bombardment mass spectrometry (EI-and FAB-MS) are the spectroscopic methods most extensively used for analysing the main polyphenols naturally occurring in olive oil mill waste waters (omww) and in dropped olive leaves. EI-MS is very suitable for analysing catechol, 4-methylcatechol, tyrosol and hydroxytyrosol, the main polyphenols identified in omww. The EI method has also proved to be suitable for analysing their acetyl derivatives, but does not allow the molecular ion of diacetyltyrosol and its isomers to be detected. FAB-MS is the only adequate ionization method for detecting the molecular ion of diacetyltyrosol and is more suitable than EI-MS for analysing triacetylhydroxytyrosol. The mechanisms of fragmentation of the acetyl derivatives of tyrosol and hydroxytyrosol were determined using the EI and FAB ionization methods. In addition, FAB-MS, which was performed in both positive and negative ion modes, was shown to be the only adequate ionization method for analysing oleuropein, a phenol glucoside which occurs naturally in olive leaves. The positive ion FAB-MS was shown to be much more suitable than EI-MS for analysing the aglycone obtained following the hydrolysis of oleuropein by b-glucosidase. These results confirmed the versatility of FAB-MS for analysing low molecular weight compounds, for which EI-MS proved to be an unsatisfactory method.