A reverse-phase liquid chromatographic assay for flavin-containing monooxygenase activity

✍ Scribed by John R. Cashman; John Proudfoot

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 580 KB
• Volume: 175
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90388-0

✦ Synopsis

A rapid, convenient assay for flavin-containing monooxygenase activity is described. The method is based on direct analysis of quenched incubation mixtures by reverse-phase liquid chromatography, and utilizes p-nitrophenyl-1,3-oxathiolane as the substrate. The synthesis of the substrate and the product are described. The usefulness of p-nitrophenyl-1,3-oxathiolane S-oxide formation as a measure of flavin-containing monooxygenase activity was demonstrated using highly purified and microsomal hog and rat liver flavin-containing monooxygenase. The assay is especially useful for determining stereoselectivity of flavin-containing monooxygenase activity in small amounts of crude tissue preparations.