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A reverse-phase HPLC and fluorescence detection method for measurement of 5-hydroxytryptamine (serotonin) in Planaria

✍ Scribed by Sumiyo Umeda; Gregory W. Stagliano; Michael R. Borenstein; Robert B. Raffa


Book ID
113941047
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
124 KB
Volume
51
Category
Article
ISSN
1056-8719

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A simple and sensitive reversed-phase HPLC method with UV detection was developed and validated for the quantitation of 5-fluorouracil (5-FC) in human plasma. After acidification and salting out, 5-FU was extracted into ethyl acetate and back-extracted into a basic buffer. The extract was adjusted t