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A resin-based method for the preparation of molecular nitrogen for 15N analysis from urinary and plasma components

✍ Scribed by W.W.C. Read; R.A. Harrison; D. Halliday

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 601 KB
Volume: 123
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90442-0

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✦ Synopsis

A rapid method, suitable for the recovery of ammonia, urea, and a-amino N for "N-isotopic analysis from biological fluids is described. The method, using a cationic-exchange resin to bind ammonia from neutral solution, has been adapted to allow the subsequent direct release of molecular N for isotopic analysis. The resin method has proved to be highly reproducible, more rapid and less demanding than classical procedures, amenable to convenient sample storage and transportation, and should find ready application in the preparation of clinical, agricultural, and geochemical samples that require 15N analysis.

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