✦ LIBER ✦

A reliable, sensitive, and convenient radioactive assay for benzpyrene monooxygenase

✍ Scribed by J.W. DePierre; M.S. Moron; K.A.M. Johannesen; L. Ernster

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 853 KB
Volume: 63
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90371-1

No coin nor oath required. For personal study only.

✦ Synopsis

A new radioactive assay for benzpyrene monooxygenase has been developed, characterized, and compared to the fluorescent assay generally employed. The radioactive assay is based on a single extraction which effectively separates metabolites from remaining substrate. This assay is linear within reasonable ranges of time and protein concentration and responds as expected to inhibitors and an inducer of benzpyrene monooxygenase activity. The activity measured with the present assay is about twice that measured with the fluorescent assay. Furthermore, the radioactive assay can be scaled down so that it is at least as sensitive as the fluorescent assay.

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