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A reliable method for the use of oligonucleotides as probes in blot-hybridization experiments

✍ Scribed by Jean Paquette; Carmen Sapienza

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
459 KB
Volume
3
Category
Article
ISSN
0938-8990

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✦ Synopsis

We have developed a ligation and specific-primer radiolabeling method that allows the use of oligonucleotides as probes in blot-hybridization experiments. The major advantage of the protocol is that standard hybridization and washing conditions may be used and yield high signals and low background. The observed increase in the stability and intensity of the hybridization signals appears to result from both increased length and specific radioactivity of the hybridization probe.

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