A relatively stable antifungal peptide from buckwheat seeds with antiproliferative activity toward cancer cells

✍ Scribed by Edwin H. W. Leung; T. B. Ng

• Book ID: 105360711
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 207 KB
• Volume: 13
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.891

✦ Synopsis

Abstract

An antifungal peptide with a molecular mass of approximately 4 kDa was isolated from buckwheat seeds by using ion‐exchange chromatography on SP‐Sepharose and Q‐Sepharose, and gel filtration on Superdex peptide. The peptide was adsorbed on SP‐Sepharose in 10 mM NH~4~OAc buffer (pH 4.5) and on Q‐Sepharose in 10 mM NH~4~HCO~3~ buffer (pH 9.4), and appeared to be highly purified after these two steps. It inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC~50~ of 35 and 40 µM, respectively. Its antifungal activity was stable between 0 and 70 °C, and between pH 1.0/2.0 and 13. It inhibited proliferation of Hep G2 (hepatoma) cells, L1210 (leukemia) cells, breast cancer (MCF‐7) cells, and liver embryonic WRL 68 cells with an IC~50~ of 33, 4, 25, and 37 µM, respectively. On the other hand, the peptide was unable to evoke a mitogenic response from splenocytes or induce nitric oxide production by macrophages. It inhibited HIV‐1 reverse transcriptase with an IC~50~ of 5.5 µM. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.