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A rapid whole-cell assay for superoxide dismutase

โœ Scribed by Garry C. Whitelam; Geoffrey A. Codd

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
451 KB
Volume
121
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A procedure is described for the intact-cell assay of superoxide dismutase(s). The technique involves the use of toluene which renders the cells permeable to the necessary components of a photochemical assay for supcroxide dismutase. Whole-cell superoxide dismutase activities from a number of procaryotic and eucaryotic microorganisms compare with cell-free activities and with activities reported in the literature. Using this procedure, changing levels of superoxide dismutase are readily monitored under conditions known to modulate superoxide dismutase activity assayed in vitro. In whole cells of Escherichia cob, exogenous methyl viologen causes a marked increase in superoxide dismutase activity, whereas in the cyanobacterium,

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