A rapid urease test for presumptive identification ofCryptococcus neoformans

✍ Scribed by Cristina Elena Canteros; Laura Rodero; Maria Cristina Rivas; Graciela Davel

• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 242 KB
• Volume: 136
• Category: Article
• ISSN: 0301-486X
• DOI: 10.1007/bf00436656

✦ Synopsis

A rapid method to evidence urease activity is described. Urea hydrolysis and consequent production ammonia are detected by a chemical reaction producing a blue phenol compound (indophenol blue). Three hundred and three yeast were tested. Out of 107 urease-positive organisms detected by Christensen's Urea Agar Test (CUAT) 102 were positive by our method. No false negatives were observed by this method when testing 87 Cryptococcus strains. Ths practical screening test for presumptive identification of Cryptococcus neoformans is simple, unaffected by pH changes and requires 15 minutes to be performed.