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A RAPID ULTRAFILTRATION PROCEDURE FOR THE ESTIMATION OF CALCIUM-PROTEIN BINDING CONSTANTS

✍ Scribed by Fuchs, Franklin


Book ID
115096995
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
203 KB
Volume
4
Category
Article
ISSN
0367-8377

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Although there are many methods available for detecting ligand binding to macromolecules (l-5), equilibrium dialysis is still the most commonly used. We report here a simple variation on equilibrium dialysis that greatly shortens the time needed for the assay of the thiaminebinding protein of E. col