A rapid technique to separate DNA polyme
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Michel Duguet; Marcel MΓ©chali; Jean-Michel Rossignol
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Article
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1978
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Elsevier Science
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English
β 369 KB
Selected precipitation of proteins from cytosol of mammalian cells with polyethylene glycol (PEG) at high ionic strength allows a rapid and efficient separation of DNA polymerases-o and $3 in a single operation which can be conducted on a large scale. With this technique, 90% of the DNA polymerase-c