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A rapid technique to resolve and partially purify multiple DNA polymerases from a crude extract

✍ Scribed by Stuart E. Katz; Norman B. Hecht


Book ID
107711394
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
235 KB
Volume
53
Category
Article
ISSN
0003-2697

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A rapid technique to separate DNA polyme
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Selected precipitation of proteins from cytosol of mammalian cells with polyethylene glycol (PEG) at high ionic strength allows a rapid and efficient separation of DNA polymerases-o and $3 in a single operation which can be conducted on a large scale. With this technique, 90% of the DNA polymerase-c