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A Rapid Selective Extraction Procedure for the Outer Membrane Protein (OmpF) from Escherichia coli

โœ Scribed by Steven Arcidiacono; Michelle M. Butler; Charlene M. Mello

Book ID
115646021
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
81 KB
Volume
25
Category
Article
ISSN
1046-5928

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Gene encoding a hybrid OmpF-PhoE pore pr
Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12
โœ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat