Rapid screening for plasmid DNA
β
Hughes, Colin ;Meynell, G. G.
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Article
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1977
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Springer
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English
β 441 KB
A procedure is described for demonstrating plasmid DNA and its molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each DNA species is then visualized as a discrete fluorescent band when the centrifug