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A rapid screening procedure for staphylococcal plasmids

✍ Scribed by Nahaie, Mohammad R.; Goodfellow, Michael; Harwood, Colin R.


Book ID
125425436
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
540 KB
Volume
2
Category
Article
ISSN
0167-7012

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Rapid screening for plasmid DNA
✍ Hughes, Colin ;Meynell, G. G. πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 441 KB

A procedure is described for demonstrating plasmid DNA and its molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each DNA species is then visualized as a discrete fluorescent band when the centrifug