A rapid microassay for detecting hepatitis B surface antigen by dot enzyme immunoassay
β Scribed by K. Matsumura; S. Wakatsuki; A. Iwasaki; R. Endo; K. Tanaka
- Book ID
- 109314148
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 285 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0378-1097
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π SIMILAR VOLUMES
The use of monoclonal antibodies in the development of a third generation microtitration plate enzyme immunoassay for the detection of hepatitis B virus (HBV) is described. These antibodies were tested either alone or in combination with polyclonal antibodies raised in rabbit. Horseradish peroxidase
## Abstract HBsAg and antiβHBs can be detected by doubleβantibody radioimmunoassay in a reliable routine overnight assay of sensitivity approaching that of solidphase immunoradiometric assay. The test requires careful standardisation and regular preparation of I^125^βHBsAg of appropriate quality, b