✦ LIBER ✦

A rapid method for the preparation of yeast for immunoelectron microscopy using lowicryl hm-20

✍ Scribed by Anderson, Wayne H. ;Thompson, Ed W. ;Zwizinski, Craig W.

Publisher: Wiley (John Wiley & Sons)
Year: 1991
Tongue: English
Weight: 633 KB
Volume: 18
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060180212

No coin nor oath required. For personal study only.

✦ Synopsis

We describe a fixation and embedding procedure for the yeast Saccharomyces cerevisiae using Lowicryl HM-20 which is rapid, gives excellent fixation, and avoids the low temperature handling normally associated with embedding in this resin. This procedure yields superior structural preservation when compared to the commonly used rapid embedding procedure which employs Lowicryl K4M. We demonstrate that sections prepared using our rapid procedure are suitable for use in immunogold labelling experiments.

📜 SIMILAR VOLUMES

A Rapid Method for the Preparation of Ye

A Rapid Method for the Preparation of Yeast Lysates That Facilitates the Immunodetection of Proteins Generated by the Yeast Two-Hybrid System

✍ Kenneth Langlands; Edward V. Prochownik 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 159 KB

A highly-efficient and environmental-fri

A highly-efficient and environmental-friendly method for the preparation of Mn(III)–Salen complexes encapsulated HMS by using microwave irradiation

✍ Zaihui Fu; Huiying Liao; Donglu Xiong; Zehui Zhang; Yan Jiang; Dulin Yin 📂 Article 📅 2007 🏛 Elsevier Science 🌐 English ⚖ 174 KB

A Rapid Method for the Preparation of 2-

A Rapid Method for the Preparation of 2-Substituted Oxazolo[4,5-b]pyridines Using Microwave-Assisted Direct Condensation Reactions.

✍ Mikko J. Myllymaeki; Ari M. P. Koskinen 📂 Article 📅 2007 🏛 John Wiley and Sons ⚖ 22 KB 👁 2 views

A rapid and specific HPLC-electrochemica

A rapid and specific HPLC-electrochemical method for the determination of endogenous 5-methyltetrahydrofolic acid in plasma using solid phase sample preparation with internal standardization

✍ M. D. Lucock; R. Hartley; R. W. Smithells 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 652 KB

Methods for the isolation and characteri

Methods for the isolation and characterization of constituents of natural products: XXI. Use of a celite-potassium methylate column for rapid preparation of methyl esters from microgram amounts of glycerides

✍ Daniel P. Schwartz 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 333 KB

Rapid method for measuring thermal condu

Rapid method for measuring thermal conductivity of rock cores and its preliminary use for finding the thermal resistance of cracks : Rock Mechanics Contributions and Challenges: Proc 31st US Symposium, Golden, 18–20 June 1990 P613–620. Publ Rotterdam: A A Balkema, 1990

✍ E. Ashworth; T. Ashworth 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 113 KB