A rapid method for the isolation of L-cell surface membranes using an aqueous two-phase polymer system
β Scribed by D. M. Brunette; J. E. Till
- Publisher
- Springer
- Year
- 1971
- Tongue
- English
- Weight
- 1019 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0022-2631
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π SIMILAR VOLUMES
## Abstract The behavior of a series of pure proteins partitioned in aqueous twoβphase systems is compared with their behavior during mild hydrophobic interaction chromatography (HIC). A simple theoretical rationale for this comparison is presented based upon solvophobic theory. Similarities were f
The principle that the antigen and the antibody prefer different phases in an aqueous two-phase system is the analytical basis of the work presented here. The antigen horseradish peroxidase, which is bound to a monoclonal antibody (mAb), is separated from free Ag in an aqueous phase system (polyethy