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A rapid method for counting cell nuclei using a particle sizer/counter

✍ Scribed by Andy A. Lin; Thang Nguyen; William M. Miller

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
187 KB
Volume
5
Category
Article
ISSN
0951-208X

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✦ Synopsis

We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer]counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 x 106 cells/mL.

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A rapid procedure has been devised to count the numbers of outer trophectoderm (TE) and inner cell mass (ICM) cells of mouse blastocysts by differentially labelling their nuclei in situ with polynucleotide-specific fluorochromes. The TE nuclei were labelled with propidium iodide (PI) by permeabilisi