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A rapid isolation procedure for dendritic cells from mouse spleen by centrifugal elutriation

✍ Scribed by Miriam A. Ossevoort; RenéE.M. Toes; Marloes L.H. De Bruijn; Cornelis J.M. Melief; Carl G. Figdor; W. Martin Kast


Book ID
115973003
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
675 KB
Volume
155
Category
Article
ISSN
0022-1759

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## Abstract The procedure of centrifugal elutriation was evaluated as a means of purifying large numbers of __in situ__ lymphocytes from enzymatically disaggregated mouse mammary tumors. The eluate obtained at a flow rate of 3.0 ml/min was optimal for high levels of lymphocyte recovery with low lev