✦ LIBER ✦

A rapid, inexpensive, quantitative, general-purpose densitometer and its application to one-dimensional gel electrophoretograms

✍ Scribed by John C. Haselgrove; Gary Lyons; Neal Rubenstein; Alan Kelly

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 848 KB
Volume: 150
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90534-2

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✦ Synopsis

We describe the construction of a densitometer based on a TV camera linked to an IBM personal computer (PC)' using commercially available components. We discuss some of the problems of accurate quantitative densitometry using low-cost video systems. and describe the manner in which absolute optical density values may be recorded. We describe a digital analysis routine designed to quantitate unresolved bands on noisy one-dimensional electrophoretograms.