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A rapid gas chromatography method for quantitation of free fatty acids, monoacyl-, diacyl-, and triacylglycerols without derivatization

✍ Scribed by Seok Joon Kwon; Seoung Yong Lee; Sang Woo Cho; Joon Shick Rhee

Book ID: 104644717
Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 359 KB
Volume: 7
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00152621

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✦ Synopsis

Quantitative

analysis of mixtures of free fatty acids, monoacyl-, diacyl-, and triacylglycerols by a simple and rapid gas chromatography method has been developed. The analysis was carried out on a short capillary column without derivatization. When the triacontane was used as an internal standard, we obtained relative response factors for each lipid class with reproducibility and accuracy. This method could be applied for not only compositional analysis of fats and oils but also monitoring lipase reactions.

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