A rapid enhanced chemiluminescent immunoassay for allergen-specific IgE antibodies

✍ Scribed by Stiles, Heather J. ;Bicknell, Helen J. ;Matthews, Jayne A.

• Publisher: John Wiley and Sons
• Year: 1986
• Weight: 425 KB
• Volume: 1
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170010203

✦ Synopsis

An enhanced chemiluminescent immunoassay is described for the measurement of allergen-specific IgE antibodies. The assay is demonstrated for pollen from four grass species. A comparison was made between results obtained by this method and those obtained by the radioallergosorbent (RAST) procedure; a high degree of correlation (r = 0.95) was found for D. glomerata specific IgE. The assay is rapid and can be carried out in under 1 hour. The advantages of the luminescent assay as compared with the RAST procedure are discussed.