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A rapid assay for pectinesterase activity which can be used as a prescreen for pectinesterase inhibitors

✍ Scribed by Ronald E. Zimmerman

Book ID
102629928
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
358 KB
Volume
85
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid assay for inhibition of pectinesterase has been developed to tGlitate large scale screening for possible inhibitors. The assay gives quantitative results which can be confirmed by other assay techniques. The assay uses an agar plate in which pectin and an indicator have been incorporated. The hydrolysis of pectin to pectinic acid by pectinesterase causes a lowering of the pH of the medium and produces a color change. The area of the color change produced by the hydrolysis of pectin is directly proportional to the activity of the pectinesterase.

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✍ Perdue, Adoline S. ;Bell, Frances ;Starnes, W.R. ;Hitchcock, Philip ;Teague, R.S πŸ“‚ Article πŸ“… 1950 πŸ› Elsevier βš– 346 KB πŸ‘ 2 views