A rapid assay for 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D 24-hydroxylase
โ Scribed by Maria Burgos-Trinidad; Alex J. Brown; Hector F. DeLuca
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 653 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0003-2697
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## Abstract The 25โhydroxyvitamin D~3~โ24โhydroxylase mRNA is tightly and reciprocally regulated by 1,25โdihydroxyvitamin D~3~ (1,25(OH)~2~D~3~) and parathyroid hormone (PTH). The upregulation of the 24โhydroxylase by 1,25(OH)~2~D~3~ is well established and occurs at the transcriptional level throu
A new, rapid assay for la-hydroxylase has been developed using 2%hydroxy-[ 1a-3H]vitamin D3 as the substrate. Using the solubilized and reconstituted chick la-hydroxylase, conversion of this substrate to 1,25-dihydroxyvitamin D3 causes the release of tritium into the aqueous medium. This 'Hz0 can be