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A rapid and simplified micromethod for subtyping varicella-zoster virus

✍ Scribed by Dr. Farhad Gharabaghi; Michèle Aymard; Pierre Trotemann; Catherine Gerdil

Book ID
102908090
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
528 KB
Volume
31
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

A simplified and rapid micromethod based on restriction endonuclease analysis of radiolabelled varicella‐zoster virus (VZV) DNA is described and applied to strains for comparison. This procedure is cheaper and less time consuming than that requiring viral nucleocapsid purification (macromethod). The micromethod is suitable for routine DNA analysis of VZV isolates, allows differentiation of vaccine strain from wild strains, and provides evidence for variability of wild strains.

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