✦ LIBER ✦
A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars
✍ Scribed by David Mirelman
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 485 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-L-Ala-o-isoGlu-L-Lys-D-Ala-o-Ala and UDP-GIcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [14C]glucose or [aH]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography.