✦ LIBER ✦

A rapid and simple estimation of cell cycle parameters by continuous labeling with bromodeoxyuridine

✍ Scribed by Dr. Kohsuke Sasaki; Tomoyuki Murakami; Manabu Takahashi

Book ID: 102134988
Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 310 KB
Volume: 8
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990080514

No coin nor oath required. For personal study only.

✦ Synopsis

The DNA synthesis time (T,) and other related cell cycle parameters were roughly estimated in HeLa cells labeled with bromodeoxyuridine (BrdUrd) for various durations by using the flow cytometrical technique. The labeling indices increased in proportion to time after addition of BrdUrd. The T, can be calculated from the slope of the regression line obtained by plotting the serial labeling indices against the labeling time and was equivalent to the value determined by fraction labeled cells in mid S-phase (FLSm) method. These parameters would be determined by only two samples labeled for different times. This simple method using BrdUrd provides rough but rapid estimation of T, and other cell cycle parameters without complicated mathematical procedures, in addition to cell cycle partition of cell populations.

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