## Abstract This assay measures picogram quantities of catechol drugs and endogenous catecholamines in body tissues and fluids. The catechols are converted to their ^3^HβOβmethyl metabolites during incubation with ^3^HβSβadenosylmethionine and the enzyme catecholβOβmethyltransferase. The radiolabel
β¦ LIBER β¦
A rapid and sensitive radioenzymatic assay for catechol estrogens in tissues
β Scribed by Steven M. Paul; Julius Axelrod
- Book ID
- 118937736
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 496 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0024-3205
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Dopamine (DA) was measured in various tissue extracts as [3H]methoxy-N-acetyldopamine after incubation with two partially purified enzymes, catechol-O-methyl transferase (EC 2.1.1.1) and N-acetyltransferase (EC 2.3.1.5), in the presence of [3H]adenosylmethionine and acetyl-CoA. This product can be s