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A rapid and sensitive method for measurement of leukotrienes based on HPLC

✍ Scribed by Björn Odlander; Hans-Erik Claesson

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
272 KB
Volume
2
Category
Article
ISSN
0269-3879

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✦ Synopsis

A rapid and sensitive method for simultaneous detection of leukotriene (LT) B,, LTC,, LTD,, LTE,, 5S,12Sdihydroxy-eicosatetraenoic acid, 6-trans LTB,, 12-epi-6-trans LTB, and the w-metabolites of LTBl has been developed. Leukocyte incubations were terminated by the addition of one volume of methanol, and the mixture was centrifuged. Analysis was then directly performed by reverse phase high performance liquid chromatography on a column packed with 3 p n Nucleosil C,, guarded by a precolumn. Quantitation of eluted compounds was carried out by UV-monitoring at 280 nm and integration of the elution profile. The sensitivity of the method was approximately 1 ng for both LTB, and LTC,. Accuracy was 101.6% (SD +9.8%) for LTB, and 99.9% (SD * 8.7%) for LTC, in the interval 5-160 ng.

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