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A rapid and sensitive assay for detection of replication-competent adenoviruses by a combination of microcarrier cell culture and quantitative PCR

✍ Scribed by Johanna A.C. Schalk; Claudette G.J.C.A. de Vries; Tom J.H. Orzechowski; Marianne G. Rots


Book ID
113767580
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
386 KB
Volume
145
Category
Article
ISSN
0166-0934

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## Abstract A simple and rapid assay for the quantification of infectious HIV‐1 in plasma was developed using short‐term culture and DNA PCR. This method, called __culture PCR__, allows detection and quantification of infectious HIV‐1 viraemia within 48 hours, and measures the number of infectious