A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue

✍ Scribed by R. Flores

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 385 KB
• Volume: 88
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90462-1

✦ Synopsis

A method for the quantitation of proteins in solution which involves the binding of bromophenol blue to proteins under acidic conditions has been developed. The binding of the dye to proteins is accompanied by the appearance of a strong absorbance at 610 nm, which is almost linear over the range of 10 to 80 pg for the seven proteins studied. The absorbance at 610 nm can be measured immediately after the mixing of the protein and dye solutions and is stable over a period of 8 hr. The method has very few interferences, most of which can be corrected for by the use of proper controls. Phenol, sodium dodecyl sulfate, and Triton X-100 (the last with some error) may be used with this assay at concentrations that produce strong interferences with similar methods using Coomassie brilliant blue G-250.