A Rapid and Highly Selective Resonance Scattering Spectral Assay for Trace Carcinoembryonic Antigen

✍ Scribed by Jian-Fu LI; Zhi-Liang JIANG; An-Ping DENG

• Book ID: 102098792
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 96 KB
• Volume: 26
• Category: Article
• ISSN: 0256-7660
• DOI: 10.1002/cjoc.200890407

✦ Synopsis

Gold nanoparticles, in size of 10 nm, were used to label monoclonal carcinoembryonic antigen antibody (CE-AAb) to obtain an immunonanogold resonance scattering (RS) spectral probe (AuCEAAb) for the measurement of trace carcinoembryonic antigen (CEA). In pH 6.8 Na 2 HPO 4 -NaH 2 PO 4 buffer solution and the presence of polyethylene glycol (PEG)-6000, the AuCEAAb combined with CEA to form the nanogold-labeled immunocomplex clusters in average size of 227 nm, which exhibited two scattering peaks at about 320 and 581 nm. The enhanced RS intensity at 581 nm (∆I 581 nm ) is proportional to CEA concentration in the range of 1.0-50.0 ng/mL, with a detection limit (DL) of 0.52 ng/mL. The immunonanogold RS assay was utilized to determine CEA in serum samples with high sensitivity, good selectivity and simplicity.