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A rapid and efficient transformation protocol for the grassBrachypodium distachyon

✍ Scribed by Pernille Christiansen; Claus Henrik Andersen; Thomas Didion; Marianne Folling; Klaus Kristian Nielsen


Book ID
106009241
Publisher
Springer
Year
2004
Tongue
English
Weight
413 KB
Volume
23
Category
Article
ISSN
0721-7714

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A method for plant regeneration via organogenesis in pea (Pisum sativum) using nodal thin cell layer segments has been developed.From 10 to 12 days old sterile pea seedlings, nodal expiants were excised from which leaves and axillary buds were removed. Shoot regeneration was consistently obtained fr