A rapid and efficient physiologic chemical method of labeling plasma proteins with indium-111

✍ Scribed by Dennis W. Wong

• Publisher: Elsevier Science
• Year: 1987
• Weight: 562 KB
• Volume: 38
• Category: Article
• ISSN: 0883-2889
• DOI: 10.1016/0883-2889(87)90072-4

✦ Synopsis

A rapid physiologic chemical method had been developed for labeling plasma proteins with "'In. Human serum albumin.

IgG, fibrinogen, transferrin, lactoferrin and the enzyme bovine thrombin had been successfully labeled with exceptionally high labeling yields of greater than 98%. In vitro bioassays confirmed that "'In-fibrinogen and "'In-thrombin retained 9699% of their biochemical activities after labeling with the radionuclide. All radiolabeled proteins were stable at 2-8°C in excess of 3 week periods without elution of the "label" or any significant loss of biological activity.