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A rapid and convenient method for specific 11C-labelling of synthetic polypeptides containing methionine

✍ Scribed by B. Långström; S. Sjöberg; U. Ragnarsson

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
302 KB
Volume
18
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

^11^C‐labelling of methionine residues in a synthetic peptide via the preparation of the corresponding protected, pure homocysteine peptide has been investigated. Complete deprotection of the peptide and specific methylation of the homocysteine residue can be performed in one step in liquid ammonia. As a first application of this method the synthesis of the tripeptide, Z‐Gly‐L‐Hcy (Bzl)‐Gly‐O‐Bzl, and its conversion to Gly‐Met‐Gly and the corresponding labelled Gly‐([^11^C]‐methyl) ‐ Met‐Gly, is reported. Starting with the protected peptide the labelling was performed in 20 ± 5 min (starting with ^11^CO~2~), yielding the labelled peptide in 92 ± 5 % radiochemical yield. Analyses and preparative LC can be performed within 6 min.

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A convenient method for the specific tritium labelling of beta-adrenoceptor antagonists
✍ Anders Arfwidsson; Göran Hallhagen; Kurt-Jürgen Hoffmann 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 French ⚖ 461 KB

## Abstract The specific tritium labelling of beta‐adrenoceptor antagonists of the general structure ArOCH~2~CH(OH)CH~2~NHR is described. The tritium label is introduced in the 2‐position in the aminohydroxypropoxy side‐chain starting from ArOCH, an intermediate in the synthesis of the unlabelled c