A Raman spectroscopic study on 5′-rGMP gel and self-aggregate

✍ Scribed by Kang Woo Kim; Sang Kyu Kim; Myung Soo Kim; Se Won Suh

• Publisher: Wiley (John Wiley & Sons)
• Year: 1986
• Tongue: English
• Weight: 510 KB
• Volume: 25
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360250415

✦ Synopsis

In order to analyze the melting behavior of 5-rGMP gel at acidic pH and self-aggregate near neutral pH we have obtained Raman spectra of aqueous solutions of 5'-rGMP at various temperatures. At low temperature the intensities of Raman peaks at 502, 585, 1083, 1179, 1322, 1366, 1487, and 1578 cm-I decrease due to the formation of ordered structure (Raman hypochromism). In contrast, the peaks a t 671, 725, 813, and 1338 cm become stronger at low temperature (Raman hyperchromism). The Raman hyperchromism of the 671-and 8 1 3 -~m -~ peaks have been explained in terms of detailed structural models. Recently, the 6 6 8 and 682cm-1 peaks in the Raman spectrum of aqueous 5'-ffiMP solution have been attributed to the guanine ring breathing vibrations in C 3and C2-endo conformers [