Protein fragment complementation assays (PCAs) based on different reporter proteins have been described as powerful tools for monitoring dynamic protein-protein interactions in living cells. The present study describes the construction of a PCA system based on genetic splitting of TEM-1 beta-lactama
A radioreceptor assay for TNFα-binding proteins
✍ Scribed by V. Miller; K. D. Muirden
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 565 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0172-8172
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A relatively quick and simple assay for hyaluronate was developed using the specific binding protein, hyaluronectin. The hyahrronectin was obtained by homogenizing the brains of Sprague-Dawley rats, and then centrifuging the homogenate. The resulting supematant was used as a source of crude hyaluron
A method for the detection and quantitation of picomole amounts of heparin-binding proteins is described. Proteins are first spotted on nitrocellulose and then incubated with "51-heparin. Binding of heparin to the proteins is detected by radioautography and quantitated by scanning densitometry; pro