✦ LIBER ✦

A radiometric enzymic method for the direct measurement of phosphorylethanolamine in tissue extracts

✍ Scribed by D.E. Leelavathi; Robert W. Guynn

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 686 KB
Volume: 72
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90529-7

No coin nor oath required. For personal study only.

✦ Synopsis

A simple, rapid and sensitive method for the determination of phosphorylethanolamine in the range of 2 to 12 nmol is described. The method is highly specific and as such can be used directly on perchloric acid extracts of tissue, eliminating the need for the preliminary concentration of the extracts and separation procedures required by previous methods. CTP:phosphorylethanolamine cytidylyltransferase (EC 2.7.7.14) required for the assay is partially purified from rat liver post-microsomal supematant by a one step gel filtration technique.

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