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A radiometric assay of pyridoxamine (pyridoxine) 5′-phosphate oxidase

✍ Scribed by LuAnn Langham; Barbara M. Garber; Daphne A. Roe; Michael N. Kazarinoff

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
469 KB
Volume
125
Category
Article
ISSN
0003-2697

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✦ Synopsis

A radiometric assay for pyridoxamine 5'-phosphate oxidase (pyridoxamine (pyridoxine) Sphosphate:02 oxidoreductase (deaminating), EC 1.4.3.5) has been developed utilizing N-(5'phosphopyridoxyl)[3H]tryptamine.

This assay is more sensitive than previously used colorimetric and fluorescent assays for this oxidase and furthermore is applicable to etythrocytes. Tritiated substrate is incubated with an enzyme sample in the presence of excess unlabeled tryptamine and the radiolabeled tryptamine product is extracted into toluene and quantitated by liquid scintillation counting.

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