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A radioimmunoassay procedure for type III procollagen: Its use in the detection of hepatic fibrosis

✍ Scribed by Michael R. Galambos; Delwood C. Collins; John T. Galambos

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 550 KB
Volume: 5
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840050109

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✦ Synopsis

Recent studies suggest that the serum level of Type I11 procollagen (PC-III) could be a valuable, noninvasive monitor of hepatic fibrogenesis. We have developed a sensitive and specific radioimmunoassay for PC-I11 procollagen isolated and purified from fetal goat skin which shows high cross-reaction for human PC-111. In a double-blind study, serum samples taken from 8 normal volunteers and 50 patients at the time of liver biopsy were assayed for PC-111. Each biopsy was reviewed without knowledge of the radioimmunoassay results, and old or active fibrosis was graded on a 0 to 3 (none, minimal, moderate, severe) scale. There was a significant difference between serum concentrations of PC-111 in normals (mean f S.D. = 72 ng per m12 8 ) as compared to patients with biopsy evidence of active fibrosis (mean f S.D. = 153 ng per ml 2 12; p < 0.001).

The serum concentration of PC-111 showed a good correlation (r = 0.58; p < 0.001) with the histological grade of active lobular fibrosis. Measurement of PC-I11 is a noninvasive test for detection of active fibrosis in the liver.

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