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A radioimmunoassay method for the rapid detection of Candida antibodies in experimental systemic candidiasis

โœ Scribed by Shyi Yi Huang; Charles W. Berry; Joseph T. Newman; William H. Cooper; Nannepaga Y. Zachariah

Publisher
Springer Netherlands
Year
1979
Tongue
English
Weight
421 KB
Volume
67
Category
Article
ISSN
0301-486X

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โœฆ Synopsis

Rabbits were employed as experimental models to evaluate a solid-phase radioimmunoassay (RIA) method for the diagnosis of systemic candidiasis. Ten rabbits were inoculated subcutaneously to mimic superficial candidiasis and were found to produce no antibodies to Candida as determined by both immunodiffusion and RIA procedures. However, 94 per cent of 18 rabbits systemically infected by intravenous injection of Candida cells were observed to produce antibody as assessed by the RIA technique. These data encourage further tests with human sera and the continued development of this RIA procedure as a useful tool in the early serodiagnosis of systemic candidiasis.

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