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A radioenzymatic assay for quinolinic acid

✍ Scribed by Alan C. Foster; Etsuo Okuno; Daniel S. Brougher; Robert Schwarcz

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
414 KB
Volume
158
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new and rapid method for the determination of the excitotoxic tryptophan metabolite quinolinic acid is based on its enzymatic conversion to nicotinic acid mononucleotide and, in a second step utilizing ['H]ATP, further to ['HI deamido-NAD. Specificity of the assay is assured by using a highly purified preparation of the specific quinolinic acid-catabolizing enzyme, quinolinic acid phosphoribosyltransferase, in the initial step. The limit of sensitivity was found to be 2.5 pmol of quinolinic acid, sufficient to conveniently determine quinolinic acid levels in small volumes of human urine and blood plasma.

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