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A radiochemical enzymatic endpoint assay for GTP and GDP

โœ Scribed by Paul F. Cerpovicz; Raymond S. Ochs

Book ID
107718930
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
641 KB
Volume
192
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES

An enzymatic method for the measurement
An enzymatic method for the measurement of GTP and GDP in biological samples
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A method is described for the analysis of GTP/GDP, or GDP alone, in a coupled enzymatic assay. Samples are pretreated with creatine kinase in the presence of phosphocreatine to remove the ADP present. Succinyl CoA synthetase is used to convert GTP to GDP in the presence of succinate and coenzyme A.

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