β Scribed by M. Minunni; M. Mascini; R.M. Carter; M.B. Jacobs; G.J. Lubrano; G.G. Guilbault
No coin nor oath required. For personal study only.
A novel, piezoelectric, quartz crystal microbalance (QCM) biosensor has been developed for the detection of Listeriu monocytogenes. The Listeria was immobilized on the gold surface of the crystals using different immobilization approaches. Using a liquid flow cell, it was possible to directly monitor the antigen (Ag)-antibody (Ab) binding without the use of a label, i.e. enzymes, radioactive compounds, etc. The calibration curve was prepared using a displacement assay with a response range from 2.5 X lo5 to 2.5 X 10' cells/crystal.
The assay was also performed in milk which was injected with the non-specific antigen Serraria or Listeria. The method is as sensitive as ELISA and results can be obtained in less than 15 min.
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A 20-mer DNA probe complementary to a part of an oncogene v-myc, having a mercaptohexyl group at the 5'-end, was immobilized on a gold electrode of a quartz crystal microbalance (QCM). The quantity of the probe immobilized on the electrode depended on the pH. Under acidic or basic immobilization con
A new method for biochemical oxygen demand (BOD) determination, which combines the series piezoelectric quartz crystal (SPQC) technique with the growth of a microorganism is presented in this paper. This method needs no immobilization of bacteria and is simple and convenient. When a calibration tech