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A quantitative method for determination of 5,6-dihydroxyindole-2-carboxylic acid using high-pressure liquid chromatography with fluorometric detection

✍ Scribed by Christer Hansson

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
434 KB
Volume
131
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for the determination of the urinary melanocyte metabolite 5,6-dihydroxyindole-2-carboxylic acid is described. The catecholic indole is adsorbed onto alumina, chromatographed on a reverse-phase octadecyl silica column, and detected by fluorometry. The sensitivity of the method permits detection of 1 pmol injected.

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