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A quantitative investigation of the interaction of copper(II) and manganese(II) with some purine bases, nucleosides and nucleotides by nuclear magnetic resonance

✍ Scribed by G.Victor Fazakerley; Graham E. Jackson; Melanie A. Phillips; Jill C. Van Niekerk


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
975 KB
Volume
35
Category
Article
ISSN
0020-1693

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✦ Synopsis


The aqueous solution interaction between Cu(II) or Mn(II) and base coordination sites on some purine bases is examined quantitatively. For Ck(II) it was found that a relatively strong chelate is formed with adenines through coordination at N-7 and the amino group (or imine group). When the C-6 substituent is an oxygen donor a chelate structure is not found and monodentate coordination at N-7 predominates. Mn(II) interacts with hypoxanthines and inosines only through formation of a chelate at N-7 and the C-6 substituent. The competition between base and phosphate for these metal ions is examined by studying the 3':5' nucleotides. @(II) interacts only with the base of cyclic AMP but ca. 50% of the ti(II) is phosphate bound with cyclic DlP and 6-chloropurine-riboside 3':5' phosphate. Mn(II) is predominantly phosphate bound with cyclic IMP and almost

wholly so with the others.